• Product name
  • Description
    Rabbit polyclonal to RBPJK
  • Specificity
    ab33065 appears to recognise a band at approximately 56 kDa in pancreatic tissue corresponding to RBPJK. However, ab33065 also recognises a band at 35 kDa, which may be due to non-specific binding.
  • Tested applications
    Suitable for: IHC-P, EMSA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from within residues 300 - 400 of Human RBPJK.

    (Peptide available as ab26982.)

  • Positive control
    • This antibody gave a positive signal in Human Pancreas tissue lysate in both Western blot and Immunohistochemistry.



Our Abpromise guarantee covers the use of ab33065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.
EMSA Use at an assay dependent concentration. PubMed: 21991380
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).


  • Function
    Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
  • Sequence similarities
    Belongs to the Su(H) family.
    Contains 1 IPT/TIG domain.
  • Cellular localization
    Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI843960 antibody
    • AOS3 antibody
    • CBF 1 antibody
    • CBF-1 antibody
    • CBF1 antibody
    • csl antibody
    • IGKJRB antibody
    • IGKJRB1 antibody
    • J kappa recombination signal binding protein antibody
    • J kappa-recombination signal-binding protein antibody
    • KBF2 antibody
    • NY REN 30 antigen antibody
    • RBP J antibody
    • RBP J kappa antibody
    • RBP JK antibody
    • RBP-J antibody
    • RBP-J kappa antibody
    • RBP-JK antibody
    • Rbpj antibody
    • RBPJK antibody
    • RBPSUH antibody
    • recombination signal binding protein for immunoglobulin kappa J region antibody
    • Recombining binding protein suppressor of hairless antibody
    • Renal carcinoma antigen NY-REN-30 antibody
    • SUH antibody
    • SUH_HUMAN antibody
    see all

Anti-RBPJK antibody images

  • Anti-RBPJK antibody (ab33065) at 1 µg/ml (Pancreas (Human) Tissue Lysate )

    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab33065 staining RBPJK in human pancreatic tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

References for Anti-RBPJK antibody (ab33065)

This product has been referenced in:
  • Nagao H  et al. RBPJ is a novel target for rhabdomyosarcoma therapy. PLoS One 7:e39268 (2012). WB ; Human . Read more (PubMed: 22792167) »
  • Bartels SJ  et al. A SILAC-Based Screen for Methyl-CpG Binding Proteins Identifies RBP-J as a DNA Methylation and Sequence-Specific Binding Protein. PLoS One 6:e25884 (2011). EMSA ; Human . Read more (PubMed: 21991380) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Jan 22 2009


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