Overview

  • Product nameAnti-RBPJK antibody
    See all RBPJK primary antibodies
  • Description
    Rabbit polyclonal to RBPJK
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 258-498 of Human RBPJK (NP_005340)

  • Positive control
    • 293T, A431, HeLa, MOLT4 and Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionTranscriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
  • Sequence similaritiesBelongs to the Su(H) family.
    Contains 1 IPT/TIG domain.
  • Cellular localizationNucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI843960 antibody
    • CBF 1 antibody
    • CBF-1 antibody
    • CBF1 antibody
    • IGKJRB1 antibody
    • J kappa recombination signal binding protein antibody
    • J kappa-recombination signal-binding protein antibody
    • KBF2 antibody
    • NY REN 30 antigen antibody
    • RBP J kappa antibody
    • RBP-J antibody
    • RBP-J kappa antibody
    • RBP-JK antibody
    • Rbpj antibody
    • RBPJK antibody
    • RBPSUH antibody
    • Recombining binding protein suppressor of hairless antibody
    • Renal carcinoma antigen NY-REN-30 antibody
    • SUH_HUMAN antibody
    see all

Anti-RBPJK antibody images

  • Anti-RBPJK antibody (ab97524) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 56 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97524 at 1/100 dilution. Lower panel is merged with DNA probe.

References for Anti-RBPJK antibody (ab97524)

ab97524 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Cartilage)
Specification Cartilage
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
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Submitted Jan 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"