Anti-RBPJK antibody (ab25949)
Key features and details
- Rabbit polyclonal to RBPJK
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-RBPJK antibody
See all RBPJK primary antibodies -
Description
Rabbit polyclonal to RBPJK -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human RBPJK.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use a concentration of 5 µg/ml.
|
|
WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
|
Notes |
---|
IP
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
Target
-
Function
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. -
Sequence similarities
Belongs to the Su(H) family.
Contains 1 IPT/TIG domain. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway. - Information by UniProt
-
Database links
- Entrez Gene: 3516 Human
- Entrez Gene: 19664 Mouse
- Omim: 147183 Human
- SwissProt: Q06330 Human
- SwissProt: P31266 Mouse
- Unigene: 479396 Human
- Unigene: 209292 Mouse
-
Alternative names
- AI843960 antibody
- AOS3 antibody
- CBF 1 antibody
see all
Images
-
All lanes : ab25949 at 1 µg/ml
Lane 1 : Testis (Human) Tissue Lysate at 20 µg
Lane 2 : Testis (Mouse) Tissue Lysate at 20 µg
Lane 3 : HeLa (Human) Whole cell lysate at 10 µg
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
-
Anti-RBPJK antibody (ab25949) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 20 µg
Secondary
Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25949 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody conjugated to HRP (ab191866), and visualised using ECL development solution ab133406.
-
RBPJK was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to RBPJK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25949.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 56kDa, non specific bands -50kDa: This extra band is heavy chain contamination; RBPJK
-
All lanes : Anti-RBPJK antibody (ab25949) at 1/500 dilution
Lane 1 : Mouse myoblast C2C12 whole cell lysate at 50 µg
Lane 2 : Mouse myoblast C2C12 whole cell lysate at 75 µg
Secondary
All lanes : HRP-conjugated Goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 1 minute
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (36)
ab25949 has been referenced in 36 publications.
- Liu MJ et al. Elevated RBP-J? and CXCL11 Expression in Colon Cancer is Associated with an Unfavorable Clinical Outcome. Cancer Manag Res 13:3651-3661 (2021). PubMed: 33981164
- Wu J et al. LINC01152 upregulates MAML2 expression to modulate the progression of glioblastoma multiforme via Notch signaling pathway. Cell Death Dis 12:115 (2021). PubMed: 33483471
- Pan X et al. LINC01123 enhances osteosarcoma cell growth by activating the Hedgehog pathway via the miR-516b-5p/Gli1 axis. Cancer Sci 112:2260-2271 (2021). PubMed: 33837611
- Zhang X et al. Linc-KILH potentiates Notch1 signaling through inhibiting KRT19 phosphorylation and promotes the malignancy of hepatocellular carcinoma. Int J Biol Sci 17:768-780 (2021). PubMed: 33767587
- Chandrakar P et al. Jagged-Notch-mediated divergence of immune cell crosstalk maintains the anti-inflammatory response in visceral leishmaniasis. J Cell Sci 134:N/A (2021). PubMed: 33589499