The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2-5µg/mg of lysate.
WB: 1/500 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRepresses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1.
Tissue specificityWidely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
Western Blot showing ab70195, at 1µg/ml, staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70195 at 3µg/ml (1 mg/IP; 1/4 of IP loaded/lane). Detection:
Chemiluminescence with an exposure time of 3 minutes.
References for Anti-RC3H1 antibody (ab70195)
has not yet been referenced specifically in any publications.
Publishing research using ab70195? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"