• Product name
  • Description
    Rabbit polyclonal to RC3H1
  • Specificity
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 500 and 550 of human RC3H1

  • Positive control
    • HeLa and 293T whole cell lysates.



Our Abpromise guarantee covers the use of ab70195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/500 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1.
    • Tissue specificity
      Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
    • Sequence similarities
      Contains 1 C3H1-type zinc finger.
      Contains 1 RING-type zinc finger.
    • Cellular localization
      Cytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA2025 antibody
      • Rc3h1 antibody
      • RC3H1_HUMAN antibody
      • RGD1563512 antibody
      • RING finger and C3H zinc finger protein 1 antibody
      • Ring Finger and CCCH type Zinc Finger Domain 1 antibody
      • RING finger protein 198 antibody
      • RNF198 antibody
      • Roquin antibody
      • RP5 1198E17.5 antibody
      • Sanroque protein antibody
      see all

    Anti-RC3H1 antibody images

    • All lanes : Anti-RC3H1 antibody (ab70195) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size : 126 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

      Exposure time : 3 minutes
    • Western Blot showing ab70195, at 1µg/ml, staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70195 at 3µg/ml (1 mg/IP; 1/4 of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 minutes.

    References for Anti-RC3H1 antibody (ab70195)

    ab70195 has not yet been referenced specifically in any publications.

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