Overview

  • Product nameAnti-RC3H1 antibody
    See all RC3H1 primary antibodies
  • Description
    Rabbit polyclonal to RC3H1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between C terminal residues 1075 and 1133 of human RC3H1 (NP_742068.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRepresses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1.
    • Tissue specificityWidely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
    • Sequence similaritiesContains 1 C3H1-type zinc finger.
      Contains 1 RING-type zinc finger.
    • Cellular localizationCytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA2025 antibody
      • Rc3h1 antibody
      • RC3H1_HUMAN antibody
      • RGD1563512 antibody
      • RING finger and C3H zinc finger protein 1 antibody
      • Ring Finger and CCCH type Zinc Finger Domain 1 antibody
      • RING finger protein 198 antibody
      • RNF198 antibody
      • Roquin antibody
      • RP5 1198E17.5 antibody
      • Sanroque protein antibody
      see all

    Anti-RC3H1 antibody images

    • All lanes : Anti-RC3H1 antibody (ab70196) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 126 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Western Blot showing Lane 2: ab70196 at 0.04µg/ml staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70196 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 1: an irrelevant antibody was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-RC3H1 antibody (ab70196)

    ab70196 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"