The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/10000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Use at 1-4 µg/mg of lysate.
FunctionComponent of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS9 subfamily.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling RC74 with ab70586 at 1/5000 (0.2µg/ml). Detection: DAB.
Western blot - RC74 antibody (ab70586)
Anti-RC74 antibody (ab70586) at 0.05 µg/ml + HeLa cell nuclear extract at 25 µg
Predicted band size : 74 kDa Observed band size : 74 kDa Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - RC74 antibody (ab70586)
Immunoprecipitation of RC74 from HeLa cell nuclear extract (5 mg for IP) with ab70586 at 4 µg/5 mg extract for IP. Detection: Coomassie stain. The identity of the bands that are RC74 was verified by mass spectrometry.
References for Anti-RC74 antibody (ab70586)
This product has been referenced in:
Xie M et al. The host Integrator complex acts in transcription-independent maturation of herpesvirus microRNA 3' ends. Genes Dev29:1552-64 (2015).
Read more (PubMed: 26220997) »