• Product nameAnti-RC74 antibody
    See all RC74 primary antibodies
  • Description
    Rabbit polyclonal to RC74
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 150 and 200 of human RC74 (NP_060720.1)

  • Positive control
    • HeLa cell nuclear extract.



Our Abpromise guarantee covers the use of ab70586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IP Use at 1-4 µg/mg of lysate.


  • FunctionComponent of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
  • Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS9 subfamily.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPSF antibody
    • CPSF2 antibody
    • CPSF2L antibody
    • FLJ10871 antibody
    • Int9 antibody
    • INT9_HUMAN antibody
    • Integrator complex subunit 9 antibody
    • INTS 9 antibody
    • INTS9 antibody
    • Protein related to CPSF subunits of 74 kDa antibody
    • RC 74 antibody
    • RC-74 antibody
    • RC74 antibody
    • Related to CPSF subunits 74 kDa antibody
    see all

Anti-RC74 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling RC74 with ab70586 at 1/5000 (0.2µg/ml). Detection: DAB.
  • Anti-RC74 antibody (ab70586) at 0.05 µg/ml + HeLa cell nuclear extract at 25 µg

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation of RC74 from HeLa cell nuclear extract (5 mg for IP) with ab70586 at 4 µg/5 mg extract for IP.
    Detection: Coomassie stain.
    The identity of the bands that are RC74 was verified by mass spectrometry.

References for Anti-RC74 antibody (ab70586)

This product has been referenced in:
  • Xie M  et al. The host Integrator complex acts in transcription-independent maturation of herpesvirus microRNA 3' ends. Genes Dev 29:1552-64 (2015). Read more (PubMed: 26220997) »

See 1 Publication for this product

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