Overview

  • Product nameAnti-RCC1 antibody
    See all RCC1 primary antibodies
  • Description
    Rabbit polyclonal to RCC1
  • Tested applicationsSuitable for: IHC-P, IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
  • Immunogen

    Recombinant full length protein (Xenopus laevis) - His-tagged and purified from E. coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab4785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 64 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • Sequence similaritiesContains 7 RCC1 repeats.
  • Post-translational
    modifications
    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • Database links
    • Alternative names
      • Cell cycle regulatory protein antibody
      • CHC 1 antibody
      • CHC1 antibody
      • Chromosome condensation 1 antibody
      • Chromosome condensation protein 1 antibody
      • Guanine nucleotide releasing protein antibody
      • HERC2 antibody
      • Ran GEF antibody
      • RanGEF antibody
      • RCC 1 antibody
      • RCC1 antibody
      • RCC1 I antibody
      • RCC1_HUMAN antibody
      • Regulator of chromosome condensation 1 antibody
      • Regulator of chromosome condensation antibody
      • SHEP1 antibody
      • SNHG3 RCC1 antibody
      • SNHG3 RCC1 readthrough transcript antibody
      see all

    Anti-RCC1 antibody images

    • ab4785 (1/100) staining RCC1 in Skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).



    • Predicted band size : 45 kDa
      ab4785 at a 1/500 dilution staining approximately 64kDa RCC 1 in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 15 seconds exposure). ab4785 at a 1/500 dilution staining approximately 64kDa RCC 1 in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 15 seconds exposure).

    References for Anti-RCC1 antibody (ab4785)

    ab4785 has not yet been referenced specifically in any publications.

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    The supplying lab let me know that, unfortunately,they do not have an IP protocol asthe researchers inthe originating lab who tested the antibody in IP haveleft that lab and the records have not been kept. I am v...

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