Overview

  • Product nameAnti-RCC1 antibody
    See all RCC1 primary antibodies
  • Description
    Mouse monoclonal to RCC1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 312-421 of Human RCC1

Properties

Applications

Our Abpromise guarantee covers the use of ab54600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
    • Sequence similaritiesContains 7 RCC1 repeats.
    • Post-translational
      modifications
      N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
    • Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell cycle regulatory protein antibody
      • CHC 1 antibody
      • CHC1 antibody
      • Chromosome condensation 1 antibody
      • Chromosome condensation protein 1 antibody
      • Guanine nucleotide releasing protein antibody
      • HERC2 antibody
      • Ran GEF antibody
      • RanGEF antibody
      • RCC 1 antibody
      • RCC1 antibody
      • RCC1 I antibody
      • RCC1_HUMAN antibody
      • Regulator of chromosome condensation 1 antibody
      • Regulator of chromosome condensation antibody
      • SHEP1 antibody
      • SNHG3 RCC1 antibody
      • SNHG3 RCC1 readthrough transcript antibody
      see all

    Anti-RCC1 antibody images

    • RCC1 antibody (ab54600) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human thyroid nodular goiter.


    • Predicted band size : 45 kDa
      RCC1 antibody (ab54600) at 1ug/lane + A-431 cell lysate at 25ug/lane.

    References for Anti-RCC1 antibody (ab54600)

    This product has been referenced in:
    • Chase GP  et al. Influenza Virus Ribonucleoprotein Complexes Gain Preferential Access to Cellular Export Machinery through Chromatin Targeting. PLoS Pathog 7:e1002187 (2011). WB ; Human . Read more (PubMed: 21909257) »
    • Graham JD  et al. DNA replication licensing and progenitor numbers are increased by progesterone in normal human breast. Endocrinology 150:3318-26 (2009). IHC-P ; Human . Read more (PubMed: 19342456) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Mouse lung)
    Specification Mouse lung
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse lung)
    Loading amount 100000 cells
    Specification Mouse lung
    Gel Running Conditions Reduced Denaturing (12.5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Breast cell line)
    Loading amount 100000 cells
    Specification Breast cell line
    Gel Running Conditions Reduced Denaturing (12.5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cells)
    Loading amount 20 µg
    Specification HeLa cells
    Gel Running Conditions Reduced Denaturing (12.5%)
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Dr. Efrat Shema

    Verified customer

    Submitted Sep 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"