The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml. IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Sequence similaritiesContains 7 RCC1 repeats.
Post-translational modificationsN-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
RCC1 antibody (ab54600) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human thyroid nodular goiter.
Western blot - RCC1 antibody (ab54600)
Predicted band size : 45 kDa RCC1 antibody (ab54600) at 1ug/lane + A-431 cell lysate at 25ug/lane.
References for Anti-RCC1 antibody (ab54600)
This product has been referenced in:
Chase GP et al. Influenza Virus Ribonucleoprotein Complexes Gain Preferential Access to Cellular Export Machinery through Chromatin Targeting. PLoS Pathog7:e1002187 (2011).
Read more (PubMed: 21909257) »
Graham JD et al. DNA replication licensing and progenitor numbers are increased by progesterone in normal human breast. Endocrinology150:3318-26 (2009).
Read more (PubMed: 19342456) »