Overview

  • Product nameAnti-RCC1 antibody
    See all RCC1 primary antibodies
  • Description
    Goat polyclonal to RCC1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human RCC1 (NP_001260.1).

  • Positive control
    • Whole cell lysate from HeLa or 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • Sequence similaritiesContains 7 RCC1 repeats.
  • Post-translational
    modifications
    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle regulatory protein antibody
    • CHC 1 antibody
    • CHC1 antibody
    • Chromosome condensation 1 antibody
    • Chromosome condensation protein 1 antibody
    • Guanine nucleotide releasing protein antibody
    • HERC2 antibody
    • Ran GEF antibody
    • RanGEF antibody
    • RCC 1 antibody
    • RCC1 antibody
    • RCC1 I antibody
    • RCC1_HUMAN antibody
    • Regulator of chromosome condensation 1 antibody
    • Regulator of chromosome condensation antibody
    • SHEP1 antibody
    • SNHG3 RCC1 antibody
    • SNHG3 RCC1 readthrough transcript antibody
    see all

Anti-RCC1 antibody images

  • All lanes : Anti-RCC1 antibody (ab70749) at 1/2000 dilution

    Lane 1 : Recombinant Human RCC1 protein (ab90625) at 0.01 µg
    Lane 2 : Recombinant Human RCC1 protein (ab90625) at 0.1 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes
  • All lanes : Anti-RCC1 antibody (ab70749) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-RCC1 antibody (ab70749)

ab70749 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70749.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"