Overview

  • Product nameAnti-RCC1 antibody
    See all RCC1 primary antibodies
  • Description
    Mouse polyclonal to RCC1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human RCC1 protein (NP_001260.1).

  • Positive control
    • WB: human colon tissue lysate and RCC1 transfected 293T cell lysate ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab88798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • Sequence similaritiesContains 7 RCC1 repeats.
  • Post-translational
    modifications
    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle regulatory protein antibody
    • CHC 1 antibody
    • CHC1 antibody
    • Chromosome condensation 1 antibody
    • Chromosome condensation protein 1 antibody
    • Guanine nucleotide releasing protein antibody
    • HERC2 antibody
    • Ran GEF antibody
    • RanGEF antibody
    • RCC 1 antibody
    • RCC1 antibody
    • RCC1 I antibody
    • RCC1_HUMAN antibody
    • Regulator of chromosome condensation 1 antibody
    • Regulator of chromosome condensation antibody
    • SHEP1 antibody
    • SNHG3 RCC1 antibody
    • SNHG3 RCC1 readthrough transcript antibody
    see all

Anti-RCC1 antibody images

  • Anti-RCC1 antibody (ab88798) at 1 µg/ml + human colon tissue lysate at 50 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-RCC1 antibody (ab88798) at 1 µg/ml

    Lane 1 : RCC1 transfected 293T cell lysae
    Lane 2 : non transfected 293T cell lysae

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • ab88798, at 10 µg/ml, staining RCC1 in paraformaldehyde fixed, permeabilised HeLa cell by Immunofluorescence.

References for Anti-RCC1 antibody (ab88798)

ab88798 has not yet been referenced specifically in any publications.

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