Anti-RCC1 antibody - Aminoterminal end, prediluted (ab53650)

Overview

  • Product nameAnti-RCC1 antibody - Aminoterminal end, prediluted
    See all RCC1 primary antibodies
  • Description
    Rabbit polyclonal to RCC1 - Aminoterminal end, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to N terminal of human RCC1

  • Positive control
    • Human colon carcinoma, A431 and Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab53650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Ready-to-use for 30 min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
    • Sequence similaritiesContains 7 RCC1 repeats.
    • Post-translational
      modifications
      N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
    • Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell cycle regulatory protein antibody
      • CHC 1 antibody
      • CHC1 antibody
      • Chromosome condensation 1 antibody
      • Chromosome condensation protein 1 antibody
      • Guanine nucleotide releasing protein antibody
      • HERC2 antibody
      • Ran GEF antibody
      • RanGEF antibody
      • RCC 1 antibody
      • RCC1 antibody
      • RCC1 I antibody
      • RCC1_HUMAN antibody
      • Regulator of chromosome condensation 1 antibody
      • Regulator of chromosome condensation antibody
      • SHEP1 antibody
      • SNHG3 RCC1 antibody
      • SNHG3 RCC1 readthrough transcript antibody
      see all

    Anti-RCC1 antibody - Aminoterminal end, prediluted images

    • ab53650, used neat, staining Human RCC1 in Colon carcinoma, using Immunohistochemistry, Formalin fixed Paraffin embedded tissue

    References for Anti-RCC1 antibody - Aminoterminal end, prediluted (ab53650)

    ab53650 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"