Overview

  • Product nameAnti-RCC1 antibody [EPR5857]
    See all RCC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5857] to RCC1
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human RCC1.

  • Positive control
    • WB: A431, HeLa, Jurkat and 293T cell lysates. IHC-P: Human bladder carcinoma tissue. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. 

    Alternative versions available:

    Anti-RCC1 antibody (Alexa Fluor® 488) [EPR5857] (ab203167)

    Anti-RCC1 antibody (Alexa Fluor® 647) [EPR5857] (ab203168)

    Anti-RCC1 antibody (HRP) [EPR5857] (ab202934)

Properties

Applications

Our Abpromise guarantee covers the use of ab109379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000.
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/500 - 1/1000.

Target

  • FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • Sequence similaritiesContains 7 RCC1 repeats.
  • Post-translational
    modifications
    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle regulatory protein antibody
    • CHC 1 antibody
    • CHC1 antibody
    • Chromosome condensation 1 antibody
    • Chromosome condensation protein 1 antibody
    • Guanine nucleotide releasing protein antibody
    • HERC2 antibody
    • Ran GEF antibody
    • RanGEF antibody
    • RCC 1 antibody
    • RCC1 antibody
    • RCC1 I antibody
    • RCC1_HUMAN antibody
    • Regulator of chromosome condensation 1 antibody
    • Regulator of chromosome condensation antibody
    • SHEP1 antibody
    • SNHG3 RCC1 antibody
    • SNHG3 RCC1 readthrough transcript antibody
    see all

Anti-RCC1 antibody [EPR5857] images

  • All lanes : Anti-RCC1 antibody [EPR5857] (ab109379) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 45 kDa
  • ab109379, at a 1/100 dilution, staining RCC1 in paraffin-embedded Human bladder carcinoma tissue
  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RCC1 (red) with ab109379 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

References for Anti-RCC1 antibody [EPR5857] (ab109379)

This product has been referenced in:
  • Li T  et al. Massive renal urothelial carcinoma with renal vein tumor thrombus, pancreatic infiltration and adrenal metastasis: A case report. Oncol Lett 12:1129-1131 (2016). IHC-P ; Human . Read more (PubMed: 27446406) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"