Overview

  • Product nameAnti-RCC2 antibody
    See all RCC2 primary antibodies
  • Description
    Rabbit polyclonal to RCC2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide (Human) corresponding to to a region between residues 1 and 50 of human RCC2. (NP_061185.1)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP Use a concentration of 2 - 5 µg/ml.

Target

  • FunctionRequired for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
  • Sequence similaritiesContains 7 RCC1 repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1470 antibody
    • Protein RCC2 antibody
    • RCC 2 antibody
    • RCC1 like protein TD 60 antibody
    • RCC1-like protein TD-60 antibody
    • RCC2 antibody
    • RCC2_HUMAN antibody
    • Regulator of chromosome condensation 2 antibody
    • TD60 antibody
    • Telophase disk protein of 60 kDa antibody
    see all

Anti-RCC2 antibody images

  • All lanes : Anti-RCC2 antibody (ab70787) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab70787 at 1 µg/ml for WB, and for IP at 3 µg/mg lysate.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-RCC2 antibody (ab70787)

ab70787 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am pleased to provide the following testing protocol for ab70787 RCC2 antibody in IP. Preparation of Cell Lysate used for Immunoprecipitation Reagents needed: PBS (Phosphate Buffered Saline, pH 7.2) NaCl 8.0 g...

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