The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Application notesIs unsuitable for IP.
FunctionRequired for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
Sequence similaritiesContains 7 RCC1 repeats.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RCC2 with ab70788 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
Western blot - RCC2 antibody (ab70788)
All lanes : Anti-RCC2 antibody (ab70788) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 56 kDa Observed band size : 56 kDa
References for Anti-RCC2 antibody (ab70788)
has not yet been referenced specifically in any publications.
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