Overview

  • Product name
  • Description
    Rabbit polyclonal to RCC2
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 475 and the C-terminus (residue 522) of human RCC2. (NP_061185.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
    • Sequence similarities
      Contains 7 RCC1 repeats.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1470 antibody
      • Protein RCC2 antibody
      • RCC 2 antibody
      • RCC1 like protein TD 60 antibody
      • RCC1-like protein TD-60 antibody
      • RCC2 antibody
      • RCC2_HUMAN antibody
      • Regulator of chromosome condensation 2 antibody
      • TD60 antibody
      • Telophase disk protein of 60 kDa antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RCC2 with ab70788 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-RCC2 antibody (ab70788) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 56 kDa
      Observed band size : 56 kDa

    References

    ab70788 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    5 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 30 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (gastric)
    Loading amount
    15 µg
    Specification
    gastric
    Gel Running Conditions
    Non-reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted May 19 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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