Overview

  • Product nameAnti-RDH10 antibody
    See all RDH10 primary antibodies
  • Description
    Rabbit polyclonal to RDH10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human RDH10.

    (Peptide available as ab90185.)

  • Positive control
    • This antibody gave a positive signal in human skeletal muscle tissue lysate and the following whole cell lysates: HepG2; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab80891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).

Target

Anti-RDH10 antibody images

  • All lanes : Anti-RDH10 antibody (ab80891) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-RDH10 antibody (ab80891)

ab80891 has not yet been referenced specifically in any publications.

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A notre connaissance, ces deuxanticorps anti-RDH10n'ontété testés qu'en Western Blot et sur échantillons humains. Toutes nos garanties d'applications et d'espèces test&...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"