Anti-RDH5 antibody (ab101457)
Key features and details
- Rabbit polyclonal to RDH5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RDH5 antibody
See all RDH5 primary antibodies -
Description
Rabbit polyclonal to RDH5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 110-246 of Human RDH5 (AAH28298).
-
Positive control
- NT2D1 whole cell lysate; A549, HeLa and Jurkat cell lysates; Cal27 xenograft
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
|
|
IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (pH8.0)
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) |
Target
-
Relevance
RDH5 encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments. -
Cellular localization
Membrane; Peripheral membrane protein -
Database links
- Entrez Gene: 281448 Cow
- Entrez Gene: 5959 Human
- Entrez Gene: 19682 Mouse
- Omim: 601617 Human
- SwissProt: Q27979 Cow
- SwissProt: Q92781 Human
- SwissProt: O55240 Mouse
-
Alternative names
- 11 cis RDH antibody
- 11 cis retinol dehydrogenase antibody
- 9 cis retinol specific dehydrogenase antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab101457 has been referenced in 2 publications.
- Carlsson E et al. Increased Rate of Retinal Pigment Epithelial Cell Migration and Pro-Angiogenic Potential Ensuing From Reduced Cystatin C Expression. Invest Ophthalmol Vis Sci 61:9 (2020). PubMed: 32049341
- Samuel W et al. Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells. Mol Vis 23:60-89 (2017). Human . PubMed: 28356702