Overview

  • Product nameAnti-RDH5 antibody
    See all RDH5 primary antibodies
  • Description
    Rabbit polyclonal to RDH5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 110-246 of Human RDH5 (AAH28298).

  • Positive control
    • NT2D1 whole cell lysate; A549, HeLa and Jurkat cell lysates; Cal27 xenograft

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (pH8.0)

Target

  • RelevanceRDH5 encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments.
  • Cellular localizationMembrane; Peripheral membrane protein
  • Database links
  • Alternative names
    • 11 cis RDH antibody
    • 11 cis retinol dehydrogenase antibody
    • 9 cis retinol specific dehydrogenase antibody
    • FLJ39337 antibody
    • FLJ97089 antibody
    • HSD17B9 antibody
    • RDH 5 antibody
    • RDH1 antibody
    • Retinol dehydrogenase 1 antibody
    • Retinol dehydrogenase 5 (11 cis/9 cis) antibody
    • Retinol dehydrogenase 5 antibody
    • SDR9C5 antibody
    • Short chain dehydrogenase/reductase family 9C member 5 antibody
    see all

Anti-RDH5 antibody images

  • Anti-RDH5 antibody (ab101457) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size : 35 kDa
  • Immunohistochemical analysis of RDH5 in paraffin-embedded Cal27 xenograft, using ab101457 at 1/100 dilution.

References for Anti-RDH5 antibody (ab101457)

ab101457 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam this morning.

As we talked about on the phone this morning, I am sending you ab106738 as a free of charge replacement, so that you can see if you still get the same molecular weight band as you did when using ab...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"