Overview

  • Product name
  • Description
    Goat polyclonal to RDH5
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    C-RDRQSLPASNAFV

    , corresponding to internal sequence amino acids 19-31 of Human RDH5 (NP_002896.2).

  • Positive control
    • Mouse eye lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab106738 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 35 kDa.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    RDH5 encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments.
  • Cellular localization
    Membrane; Peripheral membrane protein
  • Database links
  • Alternative names
    • 11 cis RDH antibody
    • 11 cis retinol dehydrogenase antibody
    • 9 cis retinol specific dehydrogenase antibody
    • FLJ39337 antibody
    • FLJ97089 antibody
    • HSD17B9 antibody
    • RDH 5 antibody
    • RDH1 antibody
    • Retinol dehydrogenase 1 antibody
    • Retinol dehydrogenase 5 (11 cis/9 cis) antibody
    • Retinol dehydrogenase 5 antibody
    • SDR9C5 antibody
    • Short chain dehydrogenase/reductase family 9C member 5 antibody
    see all

Images

  • ab106738 (5µg/ml) staining of RDH5 in paraffin embedded Human Retina following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows staining of the Ganglion Cell Layer.

  • Anti-RDH5 antibody (ab106738) at 2 µg/ml + Mouse Eye Lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Primary incubation was 1 hour.

References

ab106738 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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As we talked about on the phone this morning, I am sending you ab106738 as a free of charge replacement, so that you can see if you still get the same molecular weight band as you did when using ab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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