Overview

  • Product name
  • Description
    Goat polyclonal to RDH5
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    C-RDRQSLPASNAFV

    , corresponding to internal sequence amino acids 19-31 of Human RDH5 (NP_002896.2).

  • Positive control
    • Mouse eye lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab106738 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 35 kDa.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    RDH5 encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments.
  • Cellular localization
    Membrane; Peripheral membrane protein
  • Database links
  • Alternative names
    • 11 cis RDH antibody
    • 11 cis retinol dehydrogenase antibody
    • 9 cis retinol specific dehydrogenase antibody
    • FLJ39337 antibody
    • FLJ97089 antibody
    • HSD17B9 antibody
    • RDH 5 antibody
    • RDH1 antibody
    • Retinol dehydrogenase 1 antibody
    • Retinol dehydrogenase 5 (11 cis/9 cis) antibody
    • Retinol dehydrogenase 5 antibody
    • SDR9C5 antibody
    • Short chain dehydrogenase/reductase family 9C member 5 antibody
    see all

Anti-RDH5 antibody images

  • ab106738 (5µg/ml) staining of RDH5 in paraffin embedded Human Retina following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows staining of the Ganglion Cell Layer.

  • Anti-RDH5 antibody (ab106738) at 2 µg/ml + Mouse Eye Lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Primary incubation was 1 hour.

References for Anti-RDH5 antibody (ab106738)

ab106738 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam this morning.

As we talked about on the phone this morning, I am sending you ab106738 as a free of charge replacement, so that you can see if you still get the same molecular weight band as you did when using ab...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up