Overview

  • Product name
  • Description
    Rabbit polyclonal to REA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human REA aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q99623

  • Positive control
    • This antibody gave a positive signal in both Human Heart Mitochondrial and Human Liver tissue lysates. This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2. IHC-P: FFPE human liver tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab154992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

Target

  • Function
    Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.
  • Sequence similarities
    Belongs to the prohibitin family.
  • Developmental stage
    Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • Cellular localization
    Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell receptor associated protein BAP37 antibody
    • B-cell receptor-associated protein BAP37 antibody
    • BAP antibody
    • Bap37 antibody
    • BCAP 37 antibody
    • D prohibitin antibody
    • D-prohibitin antibody
    • p22 antibody
    • Phb2 antibody
    • PHB2_HUMAN antibody
    • PNAS 141 antibody
    • Prohibitin 2 antibody
    • Prohibitin-2 antibody
    • Repressor of estrogen receptor activity antibody
    see all

Images

  • IHC image of REA staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154992, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab154992 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab154992 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-REA antibody (ab154992) at 1 µg/ml

    Lane 1 : Human Heart Mitochondrial Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 72 kDa (possible non-specific binding)


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab154992 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab154992 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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