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DyLight® fluorochromes exhibit high fluorescence intensity, photostability and water solubility and remain fluorescent from pH 4.0 and pH 9.0. Additionally, the water solubility of the DyLight® fluorochromes allows a high dye-to-antibody ratio to be achieved without causing precipitation of conjugates.
DyLight® fluorochromes have absorption maxima ranging from 350 nm to 777 nm, covering the entire visible light spectrum. Both the absorption and emission properties of the DyLight® fluorochromes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation.
Our catalog includes secondary antibodies conjugated with DyLight® 488, DyLight® 550, DyLight® 594 and DyLight® 650.
Fig 1. Emission spectra of DyLight® fluorochromes available in our catalog.
DyLight® fluorochromes are suitable for fluorescence microscopy and flow cytometry.
|Fluorochrome||Color||Ex/Em||Spectra||ε (M-1cm-1)||Spectrally equivalent dyes|
|DyLight® 488||493/518||View graph||70K||FITC, Cy2®|
|DyLight® 550||562/576||View graph||150K||Cy3®, TRITC|
|DyLight® 594||593/618||View graph||80K||Texas Red®|
|DyLight® 650||652/672||View graph||250K||Cy5®|
Table 1. Spectral properties of DyLight® fluorochromes.
ε: Molar extinction coefficient at the absorption maximum
For further information or help, contact our Scientific Support team.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidaries. Cy® and CyDye® are trademarks of GE Healthcare Limited.