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All tags IHC Mounting media

Mounting media

Mounting a tissue specimen is essential for preserving the specimen during storage as well as for enhancing imaging quality during microscopy.

After immunochemical staining, mounting media are used to adhere a coverslip to a tissue section or cell smear. Mounting media are important to protect the specimen and staining from physical damage.  Mounting media also play an important role in improving the clarity and contrast of an image during microscopy.​

There are two categories of mounting media, organic and aqueous (or hydrophobic and hydrophilic respectively). Organic mounting media can only be used for enzymatic labels where the precipitate formed between the enzyme and the chromogen is not soluble in the organic solvents used during mounting of the tissue (e.g., diaminobenzidine (DAB)).

Aqueous mounting media are generally suitable for all enzymatic label/chromogen combinations and fluorescent labels.

Recommended mounting media for non-fluorescent imaging

Product nameTypeProduct code
ImmunoHistoMount MediumAqueousab104131,  ab104132, ab104133,  ab104134
Limonene Mounting MediumNon-aqueousab104141
Mounting MediumAqueousab64230

Recommended mounting media for fluorescent imaging

For fluorescent imaging, mounting media that preserve fluorescence in tissue and cytological smears, such as the Fluoroshield range, are used, enabling imaging of samples after prolonged storage.

Product nameTypeProduct code
Aqueous Mounting MediumAqueousab128982
BrightMount Mounting MediumAqueousab103746
BrightMount Mounting Medium (Anti-fading)Aqueousab103748
Fluoroshield Mounting MediumAqueousab104135, ab104136, ab104137, ab104138
Fluoroshield Mounting Medium With DAPIAqueousab104139, ab104140
Fluoroshield Mounting Medium With PIAqueousab104129, ab104130

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