All tags Biochemicals Protease and phosphatase inhibitor cocktails

Protease and phosphatase inhibitor cocktails

Protect your protein samples from degradation and preserve their phosphorylation state with our convenient and easy to use protease and phosphatase inhibitor cocktails.

​You can use different inhibitor combinations for your experiment, as well as water soluble & DMSO versions. Browse the guide below for more information.​

Protease inhibitor cocktails

Phosphatase inhibitor cocktails

Protease and phosphatase inhibitor cocktails

Protease inhibitor cocktails


Cell lysates contain endogenous proteases which degrade proteins and can affect sensitivity and reliability in protein expression research. It is therefore common practice to add protease inhibitors to lysate samples to protect proteins of interest. Our protease inhibitor cocktails inhibit a number of proteases including serine, cysteine and aspartic proteases. 

Kit nameKey informationInhibitors includedTarget protease/phosphatase
Protease Inhibitor Cocktail (EDTA-free)Ideal for mamalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography AEBSF hydrochloride, Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, Pepstatin AAminopeptidase B and leucine aminopeptidase,  Aspartic protease, Cysteine protease, Serine protease
Protease Inhibitor Cocktail IIGeneral use cocktailAEBSF hydrochloride, Aprotinin (bovine lung), E-64, EDTA disodium salt, Leupeptin hemisulfateCysteine protease, Metalloprotease, Serine protease
Protease Inhibitor Cocktail IIIIdeal for bacterial cell extracts using metal chelation chromatography AEBSF hydrochloride, Bestatin, E-64, EDTA disodium salt, Pepstatin A, Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Metalloprotease,  Serine protease
Protease Inhibitor Cocktail IVIdeal for fungal and yeast cell extractsAEBSF hydrochloride, E-64, Pepstatin A, o-PhenanthrolineAspartic protease, Cysteine protease, Metalloprotease, Serine protease

Phosphatase inhibitor cocktails


​Approximately 30% of known proteins can be reversibly phosphorylated in order to control a number of cellular processes. Protein kinases predominantly phosphorylate at serine, threonine and tyrosine residues - phosphatases reverse this phosphorylation. When studying these processes and pathways it is therefore essential to preserve the phosphorylation state of these proteins in your samples. Our phosphatase inhibitor cocktails inhibit a number of phosphatases including specific serine/threonine and tyrosine phosphatases as well as non-specific acid and alkaline phosphatases.

Kit nameInhibitors included

Target protease/phosphatase

Phosphatase Inhibitor Cocktail I β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrateAcid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Phosphatase Inhibitor Cocktail IIImidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrateAcid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Phosphatase Inhibitor Cocktail III(-)-p-Bromotetramisole oxalate, Cantharidin, Calyculin AAlkaline Phosphatase, Serine/threonine Phosphatase (PP1 and PP2A)
Phosphatase Inhibitor Cocktail IVβ-Glycerophosphate, Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrateAcid Phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase

Protease and phosphatase inhibitor cocktails


Combined protease and phosphatase inhibitor cocktail. Ideal for the ultimate protection of your protein samples. Available with and without EDTA, a metalloproteinase inhibitor.

Kit nameKey informationInhibitors includedTarget protease/phosphatase
Protease and Phosphatase Inhibitor CocktailAEBSF-free and mass spectroscopy compatible - water solubleAprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate, EDTA disodium saltAminopeptidase B and leucine aminopeptidase, Cysteine protease, Metalloprotease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Protease and Phosphatase Inhibitor Cocktail (EDTA-free)AEBSF-free and mass spectroscopy compatible - water solubleAprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrateAminopeptidase B and leucine aminopeptidase, Cysteine protease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase


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