ab190658 detects recombinant REBOV GP without the transmembrane region (REBOV rGPdTM). No cross reactivity to Sudan ebolavirus (SEBOV) GP, Zaire ebolavirus (ZEBOV) GP, Bundibugyo (BEBOV) GP or Marburg (MARV) GP, was observed in Western blot analysis.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 42 kDa.
REBOV GP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils.
Delta-peptide does not seem to be involved in activation of primary macrophages B
REBOV GP is a homodimer; disulfide-linked. The homodimers are linked by two disulfide bonds in a parallel orientation. Delta-peptide is a monome