Anti-Rec-8 abnormal REcombination antibody (ab52792)

Overview

  • Product nameAnti-Rec-8 abnormal REcombination antibody
  • Description
    Mouse polyclonal to Rec-8 abnormal REcombination
  • Tested applicationsSuitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Caenorhabditis elegans
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to N terminal amino acids 647-746 of Caenorhabditis elegans Rec-8 abnormal REcombination. Target sequence used to make the antibody: MRTEEELENV RRRQKSSLGV QFMRTDDLEE DTRRNRLFED EERTRDARED ELFFYSSGSL LPNNRLNIHK ELLNEAEARY PEWVNFNEFT ADHDRKKAAT.

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 90 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

IHC-FoFr 1/200. PubMed: 19696886

Target

  • RelevanceRec-8 is a Rad21/Rec8 like protein. This family represents a conserved N-terminal region found in eukaryotic cohesins of the Rad21, Rec8 and Scc1 families.
  • Database links
    • Alternative names
      • Abnormal REcombination antibody
      • rec 8 antibody

    Anti-Rec-8 abnormal REcombination antibody images

    • All lanes : Anti-Rec-8 abnormal REcombination antibody (ab52792) at 1/1000 dilution

      Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 90 kDa

    References for Anti-Rec-8 abnormal REcombination antibody (ab52792)

    This product has been referenced in:
    • Shakes DC  et al. Spermatogenesis-specific features of the meiotic program in Caenorhabditis elegans. PLoS Genet 5:e1000611 (2009). IHC-FoFr ; Caenorhabditis elegans . Read more (PubMed: 19696886) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"