Anti-REC8 (phospho S251) antibody (ab56151)

Overview

  • Product nameAnti-REC8 (phospho S251) antibody
    See all REC8 primary antibodies
  • Description
    Rabbit polyclonal to REC8 (phospho S251)
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic phospho-peptide (phospho-serine 251) corresponding to N-terminal residues of mouse REC8.

Properties

Applications

Our Abpromise guarantee covers the use of ab56151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • FunctionRequired during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
  • Tissue specificityExpressed in testis and thymus.
  • Sequence similaritiesBelongs to the rad21 family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationNucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cohesin Rec8p antibody
    • Human homolog of rad21 S. pombe antibody
    • Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family antibody
    • Meiotic recombination protein REC8 homolog antibody
    • Meiotic recombination protein REC8-like 1 antibody
    • rec8 antibody
    • REC8 homolog (yeast) antibody
    • REC8 like 1 antibody
    • REC8_HUMAN antibody
    • Rec8p antibody
    • Recombination and sister chromatid cohesion protein homolog antibody
    see all

References for Anti-REC8 (phospho S251) antibody (ab56151)

ab56151 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH 3T3)
Specification NIH 3T3
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Jan 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"