Anti-RecA antibody (ab63797)
Key features and details
- Rabbit polyclonal to RecA
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-RecA antibody -
Description
Rabbit polyclonal to RecA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length RecA (Escherichia coli) highly purified by chromatography.
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Positive control
- Extract of E.coli AB1157.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/3000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
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IP |
1/600.
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Notes |
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WB
1/3000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). |
IP
1/600. |
Target
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Relevance
RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 947170 Escherichia coli
- SwissProt: P0A7G6 Escherichia coli
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Alternative names
- DNA strand exchange and recombination protein with protease and nuclease activity antibody
- lexB antibody
- Protein recA antibody
see all
Images
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Immunoprecipitation of RecA protein from crudeextract of E. coli cells. 2 μg of ab63797 was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.
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All lanes : Anti-RecA antibody (ab63797) at 1/3000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Purified RecA
Lane 3 : Extract of E.coli AB1157
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab63797 has been referenced in 36 publications.
- Xu J et al. Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis. Nat Microbiol 7:397-410 (2022). PubMed: 35165385
- Laureti L et al. Single strand gap repair: The presynaptic phase plays a pivotal role in modulating lesion tolerance pathways. PLoS Genet 18:e1010238 (2022). PubMed: 35653392
- Henry C et al. Redox controls RecA protein activity via reversible oxidation of its methionine residues. Elife 10:N/A (2021). PubMed: 33605213
- Yasmin T et al. Distribution of Holliday junctions and repair forks during Escherichia coli DNA double-strand break repair. PLoS Genet 17:e1009717 (2021). PubMed: 34432790
- Custodio R et al. Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili. Elife 10:N/A (2021). PubMed: 34232858