• Product nameAnti-RecA antibody
  • Description
    Rabbit polyclonal to RecA
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length RecA (Escherichia coli) highly purified by chromatography.

  • Positive control
    • Extract of E.coli AB1157.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: PBS, 50% Glycerol
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab63797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
IP 1/600.


  • RelevanceRecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage.
  • Cellular localizationCytoplasmic
  • Database links
    • Alternative names
      • DNA strand exchange and recombination protein with protease and nuclease activity antibody
      • lexB antibody
      • Protein recA antibody
      • recH antibody
      • Recombinase A antibody
      see all

    Anti-RecA antibody images

    • Immunoprecipitation of RecA protein from crudeextract of E. coli cells.  2 μg of ab63797 was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.

    • All lanes : Anti-RecA antibody (ab63797) at 1/3000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Purified RecA
      Lane 3 : Extract of E.coli AB1157

      Predicted band size : 38 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    References for Anti-RecA antibody (ab63797)

    This product has been referenced in:
    • Naiman K  et al. A defect in homologous recombination leads to increased translesion synthesis in E. coli. Nucleic Acids Res 44:7691-9 (2016). Read more (PubMed: 27257075) »
    • Naiman K  et al. Chronology in lesion tolerance gives priority to genetic variability. Proc Natl Acad Sci U S A 111:5526-31 (2014). Read more (PubMed: 24706928) »

    See all 7 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 1000 µg
    Gel Running Conditions Reduced Denaturing (13)
    Sample Barley (Hordeum vulgare) Tissue lysate - other (embryos and leaves soluble fraction of thylakoids)
    Specification embryos and leaves soluble fraction of thylakoids
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Franco Lencina

    Verified customer

    Submitted Jul 29 2014

    Application Western blot
    Sample Pseudoalteromonas luteoviolacea Cell lysate - whole cell (whole cell)
    Loading amount 10 µg
    Specification whole cell
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 15 2013

    BLAST alignment of your sequence with the sequence of the immunogen for this antibody, full-length E. coli RecA, (from the UniProt entry at this link: http://www.uniprot.org/uniprot/P0A7G6) returns a result of 77% identity. Since the antibody is polycl...

    Read More

    Thank you for contacting us. It is highly likely that RecA protein of V. cholerae can be detected by immunoblotting with ab63797 because of the high sequence similarity of RecA protein between V. cholerae and E. Coli while the immunogen is whol...

    Read More