Overview

  • Product name
    Anti-RecA antibody
  • Description
    Rabbit polyclonal to RecA
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length RecA (Escherichia coli) highly purified by chromatography.

  • Positive control
    • Extract of E.coli AB1157.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
IP 1/600.

Target

  • Relevance
    RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • DNA strand exchange and recombination protein with protease and nuclease activity antibody
      • lexB antibody
      • Protein recA antibody
      • recH antibody
      • Recombinase A antibody
      see all

    Images

    • Immunoprecipitation of RecA protein from crudeextract of E. coli cells.  2 μg of ab63797 was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.

    • All lanes : Anti-RecA antibody (ab63797) at 1/3000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Purified RecA
      Lane 3 : Extract of E.coli AB1157


      Predicted band size : 38 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Irazoki O  et al. The Transient Multidrug Resistance Phenotype of Salmonella enterica Swarming Cells Is Abolished by Sub-inhibitory Concentrations of Antimicrobial Compounds. Front Microbiol 8:1360 (2017). Read more (PubMed: 28769915) »
    • Azeroglu B  et al. RecG Directs DNA Synthesis during Double-Strand Break Repair. PLoS Genet 12:e1005799 (2016). ChIP . Read more (PubMed: 26872352) »

    See all 11 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    1000 µg
    Gel Running Conditions
    Reduced Denaturing (13)
    Sample
    Barley (Hordeum vulgare) Tissue lysate - other (embryos and leaves soluble fraction of thylakoids)
    Specification
    embryos and leaves soluble fraction of thylakoids
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Franco Lencina

    Verified customer

    Submitted Jul 29 2014

    Application
    Western blot
    Sample
    Pseudoalteromonas luteoviolacea Cell lysate - whole cell (whole cell)
    Loading amount
    10 µg
    Specification
    whole cell
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 15 2013

    BLAST alignment of your sequence with the sequence of the immunogen for this antibody, full-length E. coli RecA, (from the UniProt entry at this link: http://www.uniprot.org/uniprot/P0A7G6) returns a result of 77% identity. Since the antibody is polycl...

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    Thank you for contacting us. It is highly likely that RecA protein of V. cholerae can be detected by immunoblotting with ab63797 because of the high sequence similarity of RecA protein between V. cholerae and E. Coli while the immunogen is whol...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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