• Product nameAnti-RECA1 antibody [HIS52]
  • Description
    Mouse monoclonal [HIS52] to RECA1
  • SpecificityThis antibody recognises RecA-1, a cell surface antigen which is expressed by all rat endothelial cells.
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
    Does not react with: Mouse, Sheep, Rabbit, Goat, Chicken, Guinea pig, Pig
  • Immunogen

    Tissue/ cell preparation (Rat). Stromal cells from rat lymph node



Our Abpromise guarantee covers the use of ab22492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with acetone.
IHC-P Use a concentration of 10 µg/ml.


  • RelevanceRECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.

References for Anti-RECA1 antibody [HIS52] (ab22492)

This product has been referenced in:
  • Petersen-Jones HG  et al. Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls. Am J Physiol Heart Circ Physiol 308:H592-602 (2015). Read more (PubMed: 25599570) »
  • Wu CC  et al. Human umbilical vein endothelial cells protect against hypoxic-ischemic damage in neonatal brain via stromal cell-derived factor 1/C-X-C chemokine receptor type 4. Stroke 44:1402-9 (2013). Read more (PubMed: 23449265) »

See all 8 Publications for this product

Product Wall

Thank you for your query.

The epitope recognised by ab22492 is reported to be sensitive to formaldehyde fixation (which is commonly used in IHC-P). There are publications which have used this clone successfully in IHC-P but we have never been...

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Thank you for contacting us.

ab9774 is a well characterized antibody is fully guaranteed to detect the endothelial cells. Please use the product without any hesitation.

ab22492 is also reactive against vascular endothelium so this pro...

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Thank you for confirming this.

Have agood week too.

Do not hesitate to contact us again if you need any more advice or information.

Thank you very much for this confirmation. I have added one unit of anti-RECA1 ab22492 (free of charge replacement for ab9774)to the next orderto ****.

Thank you for your reply and this precision.

I have just realised that ab9774 is not an anti-RECA1 antibody. RECA1 is the clone number of this anti-Endothelial Cell antibody.

The only 2 anti-RECA1 antibodies we have in our catalog a...

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Thank you for contacting us and sorry for the delay of our reply.

After looking at our archives, we are confident that Lee et al. ordered andused ab22492, not ab9774.

Regarding the IHC, the publication mention a use of the antibody...

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