Recombinant ß-phosphoglucomutase protein (ab58869)

Overview

  • Product nameRecombinant ß-phosphoglucomutase protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli

Associated products

Specifications

Our Abpromise guarantee covers the use of ab58869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity: >10 unit/mg solid.
  • Applications

    Functional Studies

  • FormLyophilised
  • Additional notes


    This protein is suitable for the determination of alpha-amylase in clinical analysis.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: EDTA, Lactose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionImmediately prior to use, prepare stock solution of 5mg solid/ml in cold 10mM potassium phosphate. Immediately dilute 0.1ml into 15.1 ml 10mM potassium phosphate.

General Info

  • Alternative names
    • Beta PGM
    • Beta-phosphoglucomutase
    • Glucose phosphomutase
    • Glucose phosphomutase 1
    • PGM
    see all
  • Relevanceß-phosphoglucomutase is an enzyme that transfers a phosphoryl group on a glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse. Specifically, it converts ß-D-glucose-1-phosphate to ß-D-glucose-6-phosphate. This enzyme participates in both the breakdown and synthesis of glucose. Maltose metabolism in Lactococcus lactis involves the conversion of beta-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible beta-phosphoglucomutase (beta-PGM). Alpha-PGM is expressed constitutively. ß-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. The enzyme from Lactococcus lactis has been extensively characterised including a remarkable crystal structure which traps the pentacoordinate transition state.
  • Cellular localizationCytoplasmic

References for Recombinant ß-phosphoglucomutase protein (ab58869)

ab58869 has not yet been referenced specifically in any publications.

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