The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The interaction of bacterial and recombinant A-layer protein with murine macrophages was directed at determining the effect of A-protein on intracellular events that occur in primed macrophages. This was accomplished by measuring the cytotoxic product produced by peritoneal macrophages when exposed to A-protein coated latex beads. Thioglycolate elicited macrophages exhibited a low level of activation (18% cytotoxicity) that was significantly increased (48% cytotoxicity) in the presence of latex beads. Coating of the latex beads with each of the three A-protein products resulted in an increase of cytoxicity (mean +/- SEM) from 48% to 91%.
% SDS-PAGE. ab68485 was purified by proprietary chromatographic techniques.
Purity is greater than 98.0% as determined by analysis by RP-HPLC and SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The protein was lyophylized in presence of NaHCO3 at 2:1 protein:salt ratio.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in sterile 0.4% NaHCO3.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Outer membrane protein A
RelevanceAeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein also known as OmpA.
This protein comes from the gene for the monomeric form of OmpA from achromogenic atypical Aeromonas salmonicida.
References for Recombinant Aeromomonas salmonicida OmpA protein (ab68485)
has not yet been referenced specifically in any publications.
Publishing research using ab68485? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"