The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity is 225 IU/mg. One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 µmol of ammonia per minute at pH 7.3 and 37°C.
% SDS-PAGE. Purity is greater than 96.0% as determined by RP-HPLC and SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended to reconstitute the lyophilized Asparaginase in 18M-cm H2O at 1mg/ml.
Cytoplasmic asparaginase I
L ASNase I
L ASNase II
L asparaginase 1
L asparaginase 2
L asparaginase I
L asparaginase II
L asparaginase II precursor
L asparagine amidohydrolase I
L asparagine amidohydrolase II
RelevanceAsparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity.
E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
Cellular localizationCytoplasmic, Secreted and Periplasm.
References for Recombinant Asparaginase protein (ab73439)
has not yet been referenced specifically in any publications.
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