Overview

  • Product nameRecombinant Asparaginase protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli

Specifications

Our Abpromise guarantee covers the use of ab73439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity is 225 IU/mg. One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 µmol of ammonia per minute at pH 7.3 and 37°C.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purity is greater than 96.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    No additives

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionIt is recommended to reconstitute the lyophilized Asparaginase in 18M-cm H2O at 1mg/ml.

General Info

  • Alternative names
    • AnsA
    • AnsB
    • Colaspase
    • Cytoplasmic asparaginase I
    • L ASNase I
    • L ASNase II
    • L asparaginase 1
    • L asparaginase 2
    • L asparaginase I
    • L asparaginase II
    • L asparaginase II precursor
    • L asparagine amidohydrolase I
    • L asparagine amidohydrolase II
    see all
  • RelevanceAsparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
  • Cellular localizationCytoplasmic, Secreted and Periplasm.

References for Recombinant Asparaginase protein (ab73439)

ab73439 has not yet been referenced specifically in any publications.

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