The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesUnit Definition: One Unit hydrolyzes one micromole fo p-nitrophenyl-B-D-galacotpyranoside per minute at 25deg.C, pH 7.5
Minimum Activity: >=50 units per mg dry weight
Molecular weight: 540,000
Optimum pH: 6-8.
Extinction coefficient: E(1%, 280)= 20.9
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
ReconstitutionReconstitute in 0.1 M Sodium/potassium phosphate, pH 7.0.
Beta D galactosidase
RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.