Recombinant corn CKII alpha protein (ab90272)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesCorn

Specifications

Our Abpromise guarantee covers the use of ab90272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity> 1 U/mg (1 Unit is defined as 1 micromole phosphate transferred to synthetic peptide RRRDDDSDDD per min at 30 °C).
    ab90272 is shown to be extremely salt sensitive, the highest activity is shown without salt.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purified by several chromatography steps (phosphocellulose, Superose 6, Resource Q)
  • FormLiquid
  • Additional notes


    Zea mays CKII alpha is less prone to degradation than the Human counterpart.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 500mM Sodium chloride, 25mM Tris HCl, 1mM DTT, 0.5mM PMSF, pH 8.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Casein kinase 2 alpha 1 polypeptide
    • Casein kinase II alpha 1
    • Casein kinase II alpha 1 subunit
    • Casein kinase II alpha subunit
    • Casein kinase II subunit alpha
    • CK II
    • CK II alpha
    • CK2 alpha
    • CK2 catalytic subunit alpha
    • CK2A1
    • CKII
    • CKIIalpha
    • CSK21_HUMAN
    • CSNK2A1
    see all
  • FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

References for Recombinant corn CKII alpha protein (ab90272)

ab90272 has not yet been referenced specifically in any publications.

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