Recombinant Cow Enterokinase protein (ab60893)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesCow

Specifications

Our Abpromise guarantee covers the use of ab60893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

  • Purity> 95 % SDS-PAGE.

  • FormLiquid
  • Additional notesThis product is supplied as 1 vial containing ~0.2units of protein.


    This protein can be used for fusion protein cleavage. The rare 5 amino acid recognition sequence of the enterokinase cleavage site is incorporated into a tag with the sequence: N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C Cleavage by enterokinase removes the tag from the fusion protein.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 200mM Sodium chloride, 20mM Tris HCl, 2mM Calcium chloride

General Info

  • Alternative names
    • PRSS 7
    • PRSS7
    • Enterokinase
    • Enterokinase catalytic subunit
    • Enteropeptidase
    • Enteropeptidase catalytic light chain
    • Enteropeptidase non catalytic heavy chain
    • ENTK
    • ENTK_HUMAN
    • PROENTEROKINASE
    • protease, serine, 7 (enterokinase)
    • PRSS7
    • Serine protease 7
    • TMPRSS15
    • Transmembrane protease serine 15
    see all
  • FunctionResponsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
  • Tissue specificityIntestinal brush border.
  • Involvement in diseaseDefects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 2 LDL-receptor class A domains.
    Contains 1 MAM domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SEA domain.
    Contains 1 SRCR domain.
  • Post-translational
    modifications
    The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
  • Cellular localizationMembrane.
  • Information by UniProt

References for Recombinant Cow Enterokinase protein (ab60893)

ab60893 has not yet been referenced specifically in any publications.

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The enterokinase ab60893 is the recombinant protein from bovine intestine (based on the sequence SwissProt ID P98072, http://www.uniprot.org/uniprot/P98072).

ab68332 on the other hand is the recombinan...

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Thank you for your enquiry regarding ab60893. I can confirm that this product can be used for the cleavage of His-tags from recombinant proteins. I hope this helps and if I can assist further, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"