Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Cow

Specifications

Our Abpromise guarantee covers the use of ab91596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    ATPase Activity Assay: Positive
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Form
    Liquid
  • Additional notes
    Endotoxin Concentration: < 50 EU/mg as determined by Limulus Amebocyte Lysate (LAL) gel clot assay
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 300mM Sodium chloride, 30mM Tris, 2mM DTT, 1mM EDTA, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 2410008N15Rik
    • Constitutive heat shock protein 70
    • Epididymis luminal protein 33
    • Epididymis secretory sperm binding protein Li 72p
    • Heat shock 70 kDa protein 8
    • Heat shock 70kD protein 10
    • Heat shock 70kD protein 8
    • Heat shock 70kDa protein 8
    • Heat shock cognate 71 kDa protein
    • Heat shock cognate protein 54
    • Heat shock cognate protein 71 kDa
    • Heat shock protein 8
    • Heat shock protein A8
    • Heat shock protein family A (Hsp70) member 8
    • Heat-shock70-KD protein 10, formerly
    • HEL 33
    • HEL S 72p
    • HSC54
    • HSC71
    • Hsc73
    • HSP71
    • HSP73
    • HSP7C_HUMAN
    • HSPA10
    • HSPA8
    • LAP1
    • Lipopolysaccharide associated protein 1
    • LPS associated protein
    • LPS associated protein 1
    • MGC102007
    • MGC106514
    • MGC114311
    • MGC118485
    • MGC131511
    • MGC29929
    • N-myristoyltransferase inhibitor protein 71
    • NIP71
    see all
  • Function
    Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Domain
    The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localization
    Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

References

ab91596 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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