Recombinant Cytomegalovirus Glycoprotein B (ab43040)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • Additional sequence informationThe protein contains the Cytomegalovirus (CMV) gB immunodominant regions.

Specifications

Our Abpromise guarantee covers the use of ab43040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityImmunoreactive with sera of CMV infected individuals.
  • Applications

    ELISA

    Western blot

  • Purity> 95 % SDS-PAGE.
    Sepharose derived purification.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 25mM Tris HCl, 1mM EDTA, pH 7.2

General Info

  • Alternative names
    • CMV glycoprotein B
    • Glycoprotein B
    • HCMV
    • HHV5 glycoprotein B
    • UL55
    see all
  • RelevanceHuman cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
  • Cellular localizationVirus envelope.

References for Recombinant Cytomegalovirus Glycoprotein B (ab43040)

ab43040 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. This product was made from a 57 amino acid long sequence and a GST fusion. The total molecular weight should be 32.2kDA. Therefore, please rest assured that the band you are seeing is indeed the Cytomegalovirus Glycoprot...

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This protein (ab43040: Active Cytomegalovirus Glycoprotein B protein fragment) was developed for research purposes for ELISA and Western blot applications only - but not for diagnostic or internal use in vivo. Therefore, no endotoxin tests we...

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Thank you for your inquiry.

I can confirm that the immunogen for ab43040 is from within the amino acid range 11-67. I am sorry the exact sequence is proprietary.

Unfortunately, we are not able to release the immunogen sequence for...

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Thank you for supplying that information.


I have been in contact with the lab and they suggested that maybe the PAGE sample was heated too long (5 minutes) prior to loading. It is most likely that a specific smallmolecular weight amino a...

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Thank you for contacting Abcam.


I have been in contact with the lab about your enquiry as to whether a doublet was expected on a western blot, when you ran ab43040 on a western blot. They have asked for some more information:

...

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Sorry for the late reply. The protein contains the CMV gB immunodominant regions without the signal peptide. I hope this is helpful. Please contact us again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"