Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli

Specifications

Our Abpromise guarantee covers the use of ab68279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 1MIU/mg. Unit Defenition: One unit of enzyme catalyzes hydrolysis of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • FormLiquid
  • Additional notes

    The enzymes of Pyrococcus furiosus are extremely thermostable. Consequently the DNA Polymerase from Pyrococcus furiosus (also known as Pfu DNA polymerase) can be used in the polymerase chain reaction (PCR) DNA amplification process. It hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing contaminating dUTP levels and providing the dUMP for dTTP biosynthesis.

    Enhancing PCR amplication: 50µl of Pfu PCR reaction system with 1-3u of dUTPase to amplify genomic DNA target up to 15-19 kb in length.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol (at concentration of 10 u/µl of the enzyme), 0.1% Tween 20 and 50, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.1mM EDTA, 0.1% Nonidet P40, pH 8.2

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform
    • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
    • Deoxyuridine triphosphatase
    • dut
    • DUT_HUMAN
    • dUTP nucleotidohydrolase
    • dUTP pyrophosphatase
    • dUTPase
    • FLJ20622
    see all
  • FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
  • Tissue specificityFound in a variety of tissues.
  • PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
  • Sequence similaritiesBelongs to the dUTPase family.
  • Post-translational
    modifications
    Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
  • Cellular localizationMitochondrion and Nucleus.
  • Information by UniProt

References for Recombinant DUT protein (ab68279)

ab68279 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"