Recombinant E. coli Beta lactamase TEM precursor protein (ab67672)

Overview

  • Product name
    Recombinant E. coli Beta lactamase TEM precursor protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Escherichia coli
    • Sequence
      MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
    • Additional sequence information
      Beta-Lactamase TEM precursor Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa.

Specifications

Our Abpromise guarantee covers the use of ab67672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 1,500IU/mg. Unit Definition: One unit will hydrolyze 1.0 µmole of indicated substrate per min at pH 7.0 at 25 ° C. The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • Form
    Lyophilised
  • Additional notes
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM PBS, pH 7

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized Beta Lactamase TEM precursor in sterile 18MOhms/cm water to not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • Beta-lactamase TEM
    • blaT-6
    • BLAT_ECOLX
    • IRT-4
    • Penicillinase
    • TEM-1
    • TEM-16/CAZ-7
    • TEM-2
    • TEM-24/CAZ-6
    • TEM-3
    • TEM-4
    • TEM-5
    • TEM-6
    • TEM-8/CAZ-2
    see all
  • Function
    TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
  • Sequence similarities
    Belongs to the class-A beta-lactamase family.
  • Information by UniProt

References

ab67672 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Unfortunatelywe do not have an image of this protein separated via SDS-PAGE.

I am sorry to not have more helpful information in this instance. Please feel free to contact me if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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