Recombinant E. coli Carbonic Anhydrase II protein (ab87351)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesEscherichia coli
    • SequenceMGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

Specifications

Our Abpromise guarantee covers the use of ab87351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab87351 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM Tris, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • CA 2
    • CA II
    • CA-II
    • Ca2
    • CAC
    • CAH2_HUMAN
    • CAII
    • Car 2
    • Car2
    • Carbonate dehydratase II
    • Carbonic anhydrase 2
    • Carbonic anhydrase B
    • Carbonic anhydrase C
    • Carbonic anhydrase C, formerly
    • Carbonic anhydrase II
    • Carbonic dehydratase
    • epididymis luminal protein 76
    • HEL-76
    see all
  • FunctionEssential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
  • Involvement in diseaseDefects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
  • Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant E. coli Carbonic Anhydrase II protein images

  • ab87351 on 15% SDS-PAGE (3µg)

References for Recombinant E. coli Carbonic Anhydrase II protein (ab87351)

ab87351 has not yet been referenced specifically in any publications.

Product Wall

Thanks for letting me know. I have changed this credit to a refund. You can use the same credit ID number I provided earlier.

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In this case I have issued you a "store credit" for a future purchase at Abcam. If you like I can change it to a refund.

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I am sorry that this protein did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by ...

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Thanks for your reply.

Upon re-reading your first email I did realize that you state the protein did not bind the NTA chip, which if it is His tagged it certainly should bind this chip. I apologize for this oversight. For this reason I am hap...

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Thank you for your enquiry.

Thus far the only data we have regardingab87351is that the protein is the correct amino acid sequence and a protein of the expected molecular weight can be purified and stained via commassie. This Carbonic Anhydras...

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Thank you for contacting us.

I am afraid these products are provided in Tris buffer to maintain their stability and it is the only form they are available in our catalogue.

I am very sorry I could not be more helpful, and appreciate...

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Thank you for contacting us.

I received the following answers from the lab to your questions:

1) Do you have a protocol and buffer information for using this His-tagged protein on a Ni++ column?

- We regrettably do not sh...

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Thank you for contacting Abcam regarding ab87351.


Regrettably, our protein's biological activity hasn't been tested yet. I should mention that it hasn't been exposed to denaturing agents, so it is possible that it's bioactive, but we ca...

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