The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Cpn10 gene was amplified by PCR from E.coli and cloned into an expression vector. This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 10% Glycerol, 100M Sodium chloride, 5mM DTT, 25mM Tris HCl, pH 7.5
10 kDa chaperonin
10 kDa heat shock protein mitochondrial
10 kDa heat shock protein, mitochondrial
Chaperonin 10 homolog
Early pregnancy factor
Heat shock 10kD protein 1 chaperonin 10
Heat shock 10kDa protein 1
Heat shock 10kDa protein 1 chaperonin 10
Heat-shock 10-kD protein
FunctionEukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
Sequence similaritiesBelongs to the GroES chaperonin family.