Recombinant E. coli Disulphide oxidoreductase protein (ab73431)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Escherichia coli
    • Sequence
      The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Lys-Ala-Trp.
    • Molecular weight
      23 kDa
    • Additional sequence information
      Disulfide Oxidoreductase produced in E.Coli is a periplasmic protein isolated from E. coli, containing 208 amino acids having a molecular mass of 23,149 Dalton.

Specifications

Our Abpromise guarantee covers the use of ab73431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE and was purified by proprietary chromatographic techniques.
  • Form
    Lyophilised
  • Additional notes
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium phosphate, 100mM Sodium chloride

  • Reconstitution
    Reconstitute in sterile 18MOhm/cm H2O to not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.dsbA

General Info

  • Alternative names
    • dsbA
    • periplasmic protein disulfide isomerase I
    • Thiol disulfide interchange protein dsbA
  • Relevance
    Disulphide oxidoreductase (DsbA) is the major oxidase responsible for generation of disulfide bonds in proteins of E. coli envelope. It is a member of the thioredoxin superfamily. DsbA introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.
  • Cellular localization
    Periplasm

References

ab73431 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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