Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesEscherichia coli

Specifications

Our Abpromise guarantee covers the use of ab91598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityProtein Refolding Assay: Positive Tested positive for its ability to stimulate DnaK's protein-refolding activity in the presence of GrpE.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    > 90% pure as determined by SDS-PAGE and Western Blot analysis
  • FormLiquid
  • Additional notesDnaJ has a tendency to aggregate in solution with ionic strength lower than 0.1 M KCl. Where lower salt conditions are required, addition of 0.05% Triton-X100 or Brij 58 is recommended.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 5mM DTT, 25mM HEPES, 200mM Potassium chloride, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Chaperone Hsp40 co chaperone with DnaK
    • Chaperone protein dnaJ
    • ECK0015
    • faa
    • groP
    • grpC
    • Heat shock protein J
    • HSP40
    • JW0014
    see all
  • RelevanceThe E. coli heat-shock protein DnaJ has been implicated in protein folding and protein complex dissociation. DnaJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues.
  • Cellular localizationCytoplasmic

Recombinant E. coli DnaJ protein images

  • SDS-PAGE Analysis:
    Lane 1: Molecular weight markers
    Lane 2: 0.5 µg ab91598
    Lane 3: 1 µg ab91598
    Lane 4: 2 µg ab91598
    Lane 5: 5 µg ab91598
  • All lanes : DnaJ Polyclonal Antibody

    Lane 1 : Molecular weight markers
    Lane 2 : ab91598 at 0.1 µg

References for Recombinant E. coli DnaJ protein (ab91598)

ab91598 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"