Recombinant E. coli Hsp40 protein (ab91598)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant E. coli Hsp40 protein
See all Hsp40 proteins and peptides -
Biological activity
Protein Refolding Assay: Positive Tested positive for its ability to stimulate DnaK's protein-refolding activity in the presence of GrpE.
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Purity
> 90 % SDS-PAGE.
> 90% pure as determined by SDS-PAGE and Western Blot analysis -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli
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Description
Recombinant E. coli Hsp40 protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab91598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
DnaJ has a tendency to aggregate in solution with ionic strength lower than 0.1 M KCl. Where lower salt conditions are required, addition of 0.05% Triton-X100 or Brij 58 is recommended. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 1.5% Potassium chloride, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.595% HEPES, 10% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DnaJ (Hsp40) homolog subfmaily B member 1
- DNAJ 1
- DNAJ B1
see all -
Function
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. -
Sequence similarities
Contains 1 J domain. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab91598 has not yet been referenced specifically in any publications.