Recombinant E. coli groEL protein (ab113176)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP0A6F6
    • SpeciesEscherichia coli
    • SequenceMAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM
    • Molecular weight60 kDa

Specifications

Our Abpromise guarantee covers the use of ab113176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    ELISA

  • Purity> 90 % SDS-PAGE.
    ab113176 is purified by multi-step chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.58% Sodium chloride, 0.08% DTT, 0.79% Tris HCl, 0.1% Magnesium chloride

General Info

  • Alternative names
    • 60 kDa chaperonin
    • groEL protein
    • groL
    • mopA
    • Protein Cpn60
    see all
  • RelevanceThe bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner.
  • Cellular localizationCytoplasmic

Recombinant E. coli groEL protein images

  • SDS-PAGE analysis
    Lane 1: MW marker
    Lane 2: 0.5 µg of ab113176 (groEL protein)
    Lane 3: 1 µg of ab113176 (groEL protein)
    Lane 4: 2 µg of ab113176 (groEL protein)
    Lane 5: 5 µg of ab113176 (groEL protein)
    Detected by Coomassie Stain.
  • Western blot analysis
    Lane 1: MW marker
    Lane 2: 100 ng of groEL protein (ab113176) probed with an anti-groEL monoclonal antibody.

References for Recombinant E. coli groEL protein (ab113176)

ab113176 has not yet been referenced specifically in any publications.

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