Recombinant E. coli groES protein (ab86440)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant E. coli groES protein -
Purity
> 95 % SDS-PAGE.
abb86440 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA
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Description
Recombinant E. coli groES protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab86440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Constituents: 0.077% DTT, 0.395% Tris HCl, 10% Glycerol, 0.58% Sodium chloride
General Info
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Alternative names
- 10 kDa chaperonin
- Co-chaperonin GroES
- groES protein
see all -
Relevance
GroEL and GroES are E.coli chaperones, which are homologs of the mitochondrial chaperones Hsp60 and Hsp10 respectively. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding in an ATP-dependent mechanism. Binding of substrate protein, in addition to binding of ATP, induces a conformational change that allows association of this binary complex with a separate lid structure, GroES. -
Cellular localization
Cytoplasmic
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab86440 has been referenced in 1 publication.
- Goemans CV et al. CnoX Is a Chaperedoxin: A Holdase that Protects Its Substrates from Irreversible Oxidation. Mol Cell 70:614-627.e7 (2018). PubMed: 29754824