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Source Escherichia coli
Amino Acid Sequence
Species Escherichia coli
Sequence MGSSHHHHHHSSGLVPRGSHMTVQTSKNPQVDIAEDNAFFPSEYSLSQYT SPVSDLDGVDYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLL PLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNP KKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRF VISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTPEIGYMPGHLTWY FGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQEMLAA YAG
Molecular weight 33 kDa including tags
Amino acids 1 to 283
Tags His tag N-Terminus Specifications
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity > 95
% SDS-PAGE. ab106879 was purified using conventional chromatography techniques.
Concentration information loading... Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
Chaperone protein hchA
Function Uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. It rapidly releases them once stress has abated.
Sequence similarities Belongs to the peptidase C56 family. HchA subfamily.
Domain Consists of a large A domain and a smaller P domain connected by a linker. The thermally induced motion of the flexible linker-loop region leads to the uncovering of a high-affinity substrate-binding site that is essential to capture nonnative proteins at high temperatures.
Cellular localization Cytoplasm.
Information by UniProt
E. coli hchA protein images
References for Recombinant
E. coli hchA protein (ab106879)
has not yet been referenced specifically in any publications.
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