Recombinant E. coli hchA protein (ab106879)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Escherichia coli
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMTVQTSKNPQVDIAEDNAFFPSEYSLSQYT SPVSDLDGVDYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLL PLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNP KKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRF VISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTPEIGYMPGHLTWY FGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQEMLAA YAG
    • Molecular weight
      33 kDa including tags
    • Amino acids
      1 to 283
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab106879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab106879 was purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • Chaperone protein hchA
    • EcHsp31
    • ECK1963
    • hchA
    • HCHA_ECOLI
    • Hsp31
    • Hsp31 molecular chaperone
    • JW1950
    • YedU
    • YzzC
    see all
  • Function
    Uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. It rapidly releases them once stress has abated.
  • Sequence similarities
    Belongs to the peptidase C56 family. HchA subfamily.
  • Domain
    Consists of a large A domain and a smaller P domain connected by a linker. The thermally induced motion of the flexible linker-loop region leads to the uncovering of a high-affinity substrate-binding site that is essential to capture nonnative proteins at high temperatures.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3 µg ab106879.

References

ab106879 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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