The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. highly purified by several steps of chromatography
This protein can be used in:
1) Studies on the mechanism of E. coli SOS response.
2) Used as an antigen for positive control in Western blotting to confirm that the Bait construct is expressed stably in the nucleus as protein of the expected size in the yeast two-hybrid method using the lexA gene.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
E. coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence (TACTGTATATATATACAGTA). LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As the result, the expression of genes belonging to the SOS regulon is induced, and DNA repair ability and mutagenic activity in the cells are enhanced.
SDS-PAGE - Recombinant E. coli LexA protein (ab63816)
SDS-PAGE analysis of Recombinant E. coli LexA protein (ab63816).
SDS-PAGE - LexA protein (ab63816)
SDS Page analysis of ab63816
This product has been referenced in:
Bunnell BE et al. Zinc blocks SOS-induced antibiotic resistance via inhibition of RecA in Escherichia coli. PLoS One12:e0178303 (2017).
Read more (PubMed: 28542496) »