Recombinant E. coli Nitroreductase protein (ab59673)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesEscherichia coli
    • SequenceMTPTIELICG HRSIRHFTDE PISEAQREAI INSARATSSS SFLQCSSIIR ITDKALREEL VTLTGGQKHV AQAAEFWVFC ADFNRHLQIC PDAQLGLAEQ LLLGVVDTAM MAQNALIAAE SLGLGGVYIG GLRNNIEAVT KLLKLPQHVL PLFGLCLGWP ADNPDLKPRL PASILVHENS YQPLDKGALA QYDEQLAEYY LTRGSNNRRD TWSDHIRRTI IKESRPFILD YLHKQGWATR
    • Amino acids1 to 240

Specifications

Our Abpromise guarantee covers the use of ab59673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    One unit will catalyse the reduction of 1µmole of Cytochrome C per minute in the presence of Menadione substrate at pH 7.4 and 37º C.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 90 % SDS-PAGE.

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, Lactose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute the contents of the vial in 0.1ml water.

General Info

  • Alternative names
    • Dihydropteridine reductase
    • dprA
    • FMN dependent nitroreductase
    • nfnB
    • nfsI
    • ntr
    • Oxygen insensitive NAD(P)H nitroreductase
    see all
  • RelevanceNitroreductase shows the ability to reduce quinines. It is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It is also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.

References for Recombinant E. coli Nitroreductase protein (ab59673)

ab59673 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Theoretically, the assay can be adapted to 96 cell plate as well. We unfortunately do not have protocol other than what we sent; you may need to optimize the protocol for better results.

I hope this inform...

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Thank you for contacting us.

Please find attached the protcol.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your inquiry. I heard back from the supplying lab and unfortunately they do not have UV spectra for this product. The protein concentration and enzymatic activity are the only parameters tested. The vial contains 1 mg of protein, 20 mg la...

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Thank you for your reply. I have requested the UV spectra from the testing laboratory. The enzyme can be prepared in the following buffer: 10mg of BSA in 100ml of PBS pH 7.4. Further dilution information is found in the assay protocol. I have included ...

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Thank you for your reply. We have not tested the reduction of CB1954 using ab59673 nor do we sell CB1954. That note from the relevance section is taken from the Swiss Prot entry for the protein. http://www.uniprot.org/uniprot/P38489 We only have tested...

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I am sorry but I now realise I gave you incorrect information in my previous email; this is an E. Coli enzyme not human origin. The gene for the E. Coli enzyme NTR is HIS tagged and expressed in a competent E. Coli cell line. There is no EC numbe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"