Recombinant E. coli phrB protein (ab156352)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant E. coli phrB protein -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMTTHLVWFRQDLRLHDNLALAAACRNS SARVLALYIATPRQWATHNMSPRQAELINAQLNGLQIALAEKGIPLLFRE VDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVEVERALRNVVCE GFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRS SGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQNGAGEYEQQR DFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNELI WREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIV DAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLA ANNGGWQWAASTGTDAAPYFRIFNPTTQGEKFDHEGEFIRQWLPELRDVP GKVVHEPWKWAQKAGVTLDYPQPIVEHKEARVQTLAAYEAARKGK -
Predicted molecular weight
56 kDa including tags -
Amino acids
1 to 472 -
Tags
His tag N-Terminus
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Description
Recombinant E. coli phrB protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab156352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride
General Info
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Alternative names
- Deoxyribodipyrimidine photo-lyase
- DNA photolyase
- EC=4.1.99.3
- Photoreactivating enzyme
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Relevance
phrB is involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab156352 has not yet been referenced specifically in any publications.