The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. purified by methods such as chromatography
This protein may be suitable for the following applications. 1) Studies on homologous recombination mechanism. 2) For SNP analysis. 3) Incorporation to DNA circuit. 4) Recognition and identification of cross-like DNA.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Holliday junction ATP dependent DNA helicase ruvA
Holliday junction DNA helicase RuvA
RelevanceIn Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.
Recombinant E. coli RuvA protein (Active) images
SDS-PAGE - Recombinant E. coli RuvA protein (Active) (ab63819)
SDS-PAGE analysis of Recombinant E. coli RuvA protein (ab63819).
SDS-PAGE - RuvA protein (ab63819)
SDS Page analysis of ab63819
References for Recombinant E. coli RuvA protein (Active) (ab63819)
has not yet been referenced specifically in any publications.
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