The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. purified by methods such as chromatography
This protein can be used for:- 1) Studies on homologous recombination mechanism. 2) To make use of the motor protein function that specifically migrates the Holliday junction by forming a complex with RuvA (branch-migration protein).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ATP dependent DNA helicase, component of RuvABC resolvasome
Holliday junction ATP dependent DNA helicase ruvB
Holliday junction helicase subunit A
RUVB (recombination protein) homolog
RelevanceIn Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity.
In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC,RuvAB is uncoupled from RuvC and DSBs may be prevented.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
Recombinant E. coli RuvB protein (Active) images
SDS-PAGE - RuvB protein (ab63827)
SDS Page analysis of ab63827
References for Recombinant E. coli RuvB protein (Active) (ab63827)
has not yet been referenced specifically in any publications.
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